Based on the "Seed Germination" section of the Human Education Edition biology textbook, an exploratory scientific experiment was designed and conducted to investigate the effects of different temperatures and soil burial depths on seedling emergence rate and growth performance of the alpine cushion plant Arenaria oreophila. This experiment aimed to enhance students" ability to comprehensively understand and analyze seed ecology within the field of biology. The results showed that temperature and seed burial depth significantly affected both seedling emergence rate and subsequent seedling growth performance, which has important implications for predicting plant community stability in alpine subnival belts. Furthermore, the practice of this experiment demonstrates the feasibility and importance of conducting exploratory scientific experiments in biology educations in middle school stage.