To study the chemical constituents and antioxidant activity of the flavones in flowers from thePolygala fallaxHemsl. Six compounds were isolatedand purified by silica gelcolumn chromatography, Sephadex LH-20 and preparative high performance liquid chromatography (HPLC)from the n-butanolfraction of 95% ethanol extract of Polygala fallax. Combined with the 1D-NMR spectral data and literature comparison, their structures were identified as kaempferol-3-glucoside (1), quercetin-3-O-β-D-galactoside (2), quercetin-3-O-β-xylopyranoside (3), quercetin-3-O-α-L-arabinoside (4), isorhamnetin-3-O-β-D-glucopyranoside (5), rutin (6), respectively. Compounds 1-6 are obtained from the plant for the first time.The antioxidant activities of compounds were evaluated by using FRAP methods. The results showed that all compounds had better antioxidant capacity.Among of them, total antioxidant activity of compound 4 was up to 4.533 mmol/g(vitamin C was of 5.951 mmol/g).Thus, the overall results not only enrich the material basis of flowers of Polygala fallax Hemsl, but also provide scientific basis for its future development and utilization.